報告題目：Genome targeting with programmable nucleases in animal model generation and beyond

報 告  人：黃行許 教授

南京大學模式動物研究所 PI

報告時間🛠：7月18日上午9:00-11:00

報告地點🦊👨‍👧‍👧：閔行校區生物藥學樓1-105室

聯 系  人：吳雨澤   This e-mail address is being protected from spambots. You need JavaScript enabled to view it.

研究方向：

1、建立不同基因工程小鼠模型研究配子發生的調控；

2、結合利用同源重組技術👩🏻‍🦲、鋅指蛋白酶技術、RNA幹擾技術、iPS技術進行不同動物的基因組改造✸。

代表性論文：

1. Wang L, Zhang J, Duan J, Gao X, Zhu W, Song C, Yang L, Zhang J, Li G, Ci W, Li W, Zhou Q, Tang F, He C, Huang X*, Liu J*. Programming and inheritance of parental DNA methylome in mammal. Cell 2014, 157(4): 979-991.

2. Shen B, Zhang J, Zhang W, Zhou J, Wang J, Chen L, Wang L, Hogkins A, Iyer V, Huang X*, Skarnes W*. Efficient genome modification in mice by CRISPR/Cas9 nickase without off-target effects. Nature Methods 2014, 11(4): 399-402.

3. Niu Y, Shen B, Cui Y, Chen Y, Wang J, Wang L, Kang Y, Zhao X, Si W, Li W, Xiang AP, Zhou J, Guo X, Si C, Hu B, Dong G, Wang H, Zhou Z, Li T, Tan T, Pu X, Wang F, Ji S, Zhou Q, Huang X*, Ji W*, Sha J*. Generation of gene-modified cynomolgus monkey via Cas9/RNA-mediated gene targeting in one-cell embryos. Cell 2014, 156(4): 836-843.

4. Ma Y, Zhang X, Shen B, Lu Y, Chen W, Ma J, Bai L, Huang X*, Zhang L*. Generating rats with conditional alleles using CRISPR/Cas9. Cell Research 2014, 24(1): 122-125.

5. Shen B, Zhang J, Wu H, Wang J, Ma K, Li Z, Zhang X, Zhang P, Huang X*. Mouse gene knockout by Cas9/RNA-mediated site-specific gene targeting. Cell Research 2013, 23(5): 720-723.